Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.

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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Pricing and Availability
Specifications

Flow Rate	4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type	Pre-packed Column
Content And Storage	4°C to 30°C
Description	HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Material	Polypropylene
Support Type	6% Highly Cross-linked Agarose

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Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

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Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Pricing and Availability
Specifications

Flow Rate	<20 mL/min.
Type	Pre-packed Column
Content And Storage	4°C to 30°C, 20% Ethanol
Diameter (English) Inner	0.6 in.
Description	HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Diameter (Metric) Inner	16 mm
Material	Polypropylene
Support Type	6% Cross-linked Agarose

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Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

Pricing and Availability
Specifications

Type	Agarose
Stationary Phase	Ni-NTA
Content And Storage	The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni²⁺ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.
Form	Suspension
Column Type	Affinity Column

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Thermo Scientific™ HisPur™ Ni-NTA Chromatography Cartridges, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Pricing and Availability
Specifications

Stationary Phase	Divalent Nickel, Metal Chelate
Type	Chromatography Cartridge
Target	His-Tagged Protein
Content And Storage	Store between 4°C and 8°C. Do not freeze.
Column Type	Affinity, Agarose Resin
Product Line	HisPur™

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Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

Pricing and Availability
Specifications

dimMatrix	Magnetic Agarose

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Thermo Scientific™ Detergent Removal Spin Columns & Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.

Pricing and Availability
Specifications

Stationary Phase	Proprietary Ligand
Column Type	Proprietary Resin, Affinity
Purification Target	Detergent Removal

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Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Pricing and Availability
Specifications

Buffer	Lysis Buffer
Shipping Condition	Ambient
Form	Liquid
Product Type	Nuclear and Cytoplasmic Extraction Reagent
Reagent Type	Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
Product Line	NE-PER™

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Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Pricing and Availability
Specifications

Stationary Phase	Divalent Nickel, Metal Chelate
Type	Agarose
Target	His-Tagged Protein
Content And Storage	Store at 4°C
Format	Bottle
Form	Liquid Suspension
Column Type	Affinity, Agarose Resin
Capacity (Metric)	20 mg His-Tagged GFP Binding per mL Resin
Product Line	HisPur™

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Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation of DYKDDDDK-tagged proteins

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Stationary Phase	Anti-DYKDDDDK monoclonal antibodies
Format	Liquid
Form	Liquid
Product Type	Affinity Resin
dimMatrix	Affinity Resin
Product Line	Pierce™

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Thermo Scientific™ AminoLink™ Plus Immobilization Kit, 2 mL

Covalently couple antibodies and other proteins via primary amines (-NH2) for affinity purification with these aldehyde-activated agarose beads.

Pricing and Availability
Specifications

Stationary Phase	Aldehyde
Type	Immobilization Kit
Target	Antibody, Proteins through Primary Amine
Format	Spin Column
Column Type	Affinity, Agarose Resin
Capacity (Metric)	2 to 40 mg of protein Immobilization per Column
Sufficient For	2 to 40 mg of Protein per Column
Product Line	AminoLink™

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Thermo Scientific™ AminoLink™ Coupling Resin

Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.

Pricing and Availability
Specifications

Stationary Phase	Aldehyde
Type	Resin
Target	Antibody, Proteins through Primary Amine
Content And Storage	Store at 4°C.
Format	Bottle
Column Type	Affinity, Agarose Resin
Product Line	AminoLink™

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

Pricing and Availability
Specifications

Height (Metric)	25 mm
Column Type	Affinity Column
For Use With (Application)	Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™

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Thermo Scientific™ ActivX™ Desthiobiotin-FP Serine Hydrolase Probe

ActivX™ probes have been designed to selectively label and enrich serine hydrolases through tagged fluorophosphonate to assess enzyme activity. ActivX Serine Hydrolase Probes covalently label serine hydrolase using fluorophosphonate conjugated with either desthiobiotin or azide.

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Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide

Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Format	Lyophilized Powder
Form	Lyophilized Powder
Protein Form	Recombinant
Product Type	Peptides
dimMatrix	Affinity Resin
For Use With (Application)	Protein Purification and Isolation
Product Line	Pierce™

Protein Purification Reagents and Kits

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